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dec 21, 2024
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2022-23 undergraduate catalog [archived catalog]
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cm 3321 - biochemistry lab (1) sp
the biochemistry laboratory course includes the determination of protein concentration, enzyme kinetics, sds-polyacrylamide gel electrophoresis, isoelectric focusing and protein isolation by high speed centrifugation and various chromatographic techniques. additionally, the manipulation of dna including the transformation of e.coli with a plasmid containing a foreign gene, expression of that gene, isolation of the plasmid from the bacteria and restriction analysis of the plasmid on agarose gels.
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